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中华关节外科杂志(电子版)

中华关节外科杂志(电子版) ›› 2023, Vol. 17 ›› Issue (01) : 29 -34. doi: 10.3877/cma.j.issn.1674-134X.2023.01.004

临床论著

两种微小RNA表达与痛风性关节炎免疫指标的相关性
安丽欣1,†(), 张倩1, 李昆1, 刘耔序1, 刘健1, 石蕊1   
  1. 1. 066000　秦皇岛市第一医院风湿免疫科
  • 收稿日期:2021-08-13 出版日期:2023-02-01
  • 通信作者: 安丽欣
  • 基金资助:
    秦皇岛市市级自筹项目(202101A087)

Correlation between miRNA22-3p and miRNA223-3p expression and immune markers in gouty arthritis

Lixin An1,†(), Qian Zhang1, Kun Li1, Zixu Liu1, Jian Liu1, Rui Shi1   

  1. 1. The First Hospital of Qinhuangdao Rheumatology and immunology, Qinhuangdao 066000, China
  • Received:2021-08-13 Published:2023-02-01
  • Corresponding author: Lixin An
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引用本文：

安丽欣, 张倩, 李昆, 刘耔序, 刘健, 石蕊. 两种微小RNA表达与痛风性关节炎免疫指标的相关性[J]. 中华关节外科杂志(电子版), 2023, 17(01): 29-34.

Lixin An, Qian Zhang, Kun Li, Zixu Liu, Jian Liu, Rui Shi. Correlation between miRNA22-3p and miRNA223-3p expression and immune markers in gouty arthritis[J]. Chinese Journal of Joint Surgery(Electronic Edition), 2023, 17(01): 29-34.

目的

研究miRNA22-3p、miRNA223-3p在痛风性关节炎患者中的表达水平及其炎症免疫反应调节机制。

方法

2020年1月到2021年1月在秦皇岛市第一医院诊治且符合痛风诊断标准的80例痛风患者资料纳入研究作为观察组进行回顾性分析。同期行健康体检且临床指标无明显异常，身体健康的80例患者作为对照组。排除标准：合并重要脏器系统严重疾病者；近3个月使用过激素类药物者；其他疾病所致继发性痛风性关节炎；药物诱发痛风性关节炎发作者；精神障碍疾病者等。采用独立样本t检验比较两组的miRNA22-3p、miRNA223-3p、炎性反应以及免疫指标之间的差异；Pearson相关性分析miRNA22-3p和miRNA223-3p与炎性因子的关系。

结果

观察组患者的miRNA22-3p、miRNA223-3p水平分别为(4.0±1.0)、(1.51±0.12)，显著高于对照组的(1.12±0.26)、(0.62±0.20)(t＝24.333、34.130，均为P<0.05)。观察组患者的白介素(IL)-1β、肿瘤坏死因子(TNF)-α及IL-6水平分别为(57.77±7.24)、(24.04±4.24)、(49.27±3.72)均显著高于对照组的(42.27±3.31)、(18.07±4.17)、(39.75±3.73)(t＝17.415、8.979、16.164，均为P<0.05);观察组的单核细胞趋化因子(MCP)-1、胸腺调节趋化因子(TARC)、嗜酸性粒细胞趋化因子(eotaxin)-2水平分别为(106±12)、(215±13)、(347±11)均显著高于对照组(72±12)、(159±12)、(317±12)(t＝18.095、29.356、16.713，均为P<0.05)。Pearson相关性分析显示患者的miRNA22-3p、miRNA223-3p与MCP-1、TARC、eotaxin-2、IL-1β、TNF-α以及IL-6呈现显著正相关(r＝0.609、0.659、0.545、0.610、0.551、0.571、0.684、0.668、0.671、0.542、0.534、0.458，均为P<0.05)。

结论

miRNA22-3p、miRNA223-3p在痛风性关节炎患者中的表达水平显著升高，与炎症以及免疫反应呈现正相关。

关键词: RNA，非翻译小片段, 关节炎，痛风性, 细胞因子
Objective

To investigate the expression levels of miRNA22-3p and miRNA223-3p in gouty arthritis patients and the regulatory mechanism of inflammatory immune response.

Methods

The data of 80 gout patients who met the gouty diagnosis creteria and treated in the First Hospital of Qinhuangdao from January 2020 to January 2021 were retrospectively analyzed. Other 80 healthy patients who underwent rountine health check-up during the same period without obvious abnormality in clinical indicators were enrolled as the control group. Exclusion criteria: the patients with severe diseases of the vital organs and systems; patients who used hormone drugs in the past three months; secondary gouty arthritis caused by other diseases; dryg induced gouty arthritis attacks; mental disorders. The observation group met the diagnostic criteria of gout. Independent sample t test was used to compare the differences in miRNA22-3p, miRNA223-3p, infla-mmatory response and immune indicators between the two groups. Pearson correlation analysis was applied for the relationship between inflammatory indicators and miRNA22-3p as well as miRNA223-3p.

Results

Expression levels of miRNA22-3p and miRNA223-3p in the observation group were (4.01±1.03) and (1.51±0.12), respectively, which were significantly higher than those in the control group [(1.12±0.26) and (0.62±0.20)] (t=24.333, 34.130, both P<0.05). The levels of IL-1β, TNF-α and IL-6 in the observation group were (57.77±7.24), (24.04±4.24) and (49.27±3.72), respectively, which were significantly higher than those in the control group. (42.27±3.31), (18.07±4.17), (39.75±3.73), respectively (t=17.415, 8.979, 16.164, all P<0.05). The levels of monocyte chemoattractant protein (MCP)-1, thymus and activation regulated chemokine (TARC), eotaxin-2 in the observation group were(106±12), (215±13), (347±11) which were higher than those in the control group [(72±11), (159±12), (317±12)](t=18.095, 29.356, 16.713, all P<0.05). Pearson correlation analysis showed the expressions of miRNA22-3p and miRNA223-3p were positively correlated with MCP-1, TARC, eotaxin-2, IL-1β, TNF-α and IL-6 (r=0.609, 0.659, 0.545, 0.610, 0.551, 0.571, 0.684, 0.668, 0.671, 0.542, 0.534, 0.458, all P<0.05).

Conclusion

The expression levels of mirNA22-3p and mirNA223-3p in gouty arthritis patients are significantly increased, which are positively correlated with inflammation and immune response.

Key words: RNA, small untranslated, Arthritis, gouty, Cytokines
表1 两组患者一般资料比较
Table 1 Comparison of general information between two groups of patients
组别Group 例数Case number 年龄Age[岁，(±s)] 身体质量指数Body mass index[kg/cm2，(±s)] 性别(男/女)Gender(male/female)
观察组Observation group 80 65.3±5.2 24.1±2.0 53/27
对照组Control group 80 65.3±2.1 24.1±2.5 58/22
统计值   t＝0.128 t＝0.028 χ2＝0.735
P值   >0.05 >0.05 >0.05
表2 两组患者miRNA22-3p、miRNA223-3p水平比较(±s)
Table 2 Comparison of miRNA22-3p and miRNA223-3p levels between two groups of patients
组别Group 例数Case number miRNA223-3p miRNA22-3p
观察组Observation group 80 4.01±1.03 1.51±0.12
对照组Control group 80 1.12±0.26 0.62±0.20
t值   24.333 34.130
P值   <0.001 <0.001
表3 不同状态患者的miRNA22-3p、miRNA223-3p水平比较(±s)
Table 3 Comparison of miRNA22-3p and miRNA223-3p levels in patients with different states
组别Group 例数Case number miRNA223-3p miRNA22-3p
发作亚组Episodic subgroup 42 4.3±0.9 1.7±0.3
稳定亚组Stable subgroup 38 3.7±0.8 1.3±0.6
t值   3.237 3.600
P值   0.002 0.001
表4 两组患者的炎性指标比较(±s)
Table 4 Comparison of inflammatory indicators between two groups of patients
组别Group 例数Case number IL-1β(ng/L) TNF-α(ng/L) IL-6(ng/L) NLRP3(相对表达量) caspase(相对表达量)
观察组Observation group 80 57.77±7.24 24.04±4.24 49.27±3.72 8.56±2.43 7.11±1.14
对照组Control group 80 42.27±3.31 18.07±4.17 39.75±3.73 1.59±0.44 0.98±0.13
t值   17.415 8.979 16.164 25.244 47.785
P值   <0.001 <0.001 <0.001 <0.001 <0.001
表5 两组患者的变态反应指标比较(±s)
Table 5 Comparison of allergic index between two groups of patients
组别Group 例数Case number MCP-1(ng/L) TARC(ng/L) Eotaxin-2(ng/L)
观察组Observation group 80 106±12 215±13 347±11
对照组Control group 80 72±11 159±12 317±12
t值   18.095 29.356 16.713
P值   <0.001 <0.001 <0.001
表6 miRNA22-3p、miRNA223-3p与炎性反应及变态反应指标的相关性分析
Table 6 Correlation analysis of miRNA22-3p, miRNA223-3p with inflammatory and allergic response indicators
组别Group IL-1β IL-6 MCP-1 TARC Eotaxin-2
r值 P值 r值 P值 r值 P值 r值 P值 r值 P值
miRNA223-3p 0.609 <0.001 0.545 <0.001 0.551 <0.001 0.684 <0.001 0.671 <0.001
miRNA22-3p 0.659 <0.001 0.610 <0.001 0.571 <0.001 0.668 <0.001 0.542 0.001
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