Hesperidin inhibits osteoclast differentiation mediated by titanium particles in vitro

doi:10.3877/cma.j.issn.1674-134X.2020.06.009

Abstract

Abstract:

Objective

To investigate the effects of hesperidin on titanium particles-mediated osteoclast differentiation and maturation.

Methods

Bone marrow macrophages were induced to differentiate into osteoclasts under the stimulation of M-CSF (30ng / ml) and Rankl (50ng / ml). SEM was used to observe the morphology and structure of titanium particles. Cell counting kit 8(CCK8) was used to screen the lowest effective concentration of hesperidin. The effects of hesperidin on osteoclast differentiation and maturation was determined by observing the number of tanrate resistaIlt acid phosphatase (TRAP) positive cells and observing the area of bone resorption pits by SEM. The effects of hesperidin on titanium particle-mediated osteoclast marker genes including nuclear factor of activated T-cells cytoplasmic one(NFATc1), cathepsin K(CK), TRAP was detected by real-time PCR(RT-PCR).

Results

The size of titanium particles were 1-3 μm.CCK-8 showed that hesperidin had a significant inhibitory effect on macrophages when the concentration of hesperidin exceeded 40 μmol/L (F=40.1, P<0.01). TRAP staining revealed that hesperidin significantly inhibited the differentiation of pre-osteoclasts. SEM showed that the area of the titanium particles mediated bone resorption was significantly increased, However, after the addition of 40 μmol/L hesperidin, the absorption effect was significantly reduced (t=6.1, P<0.05). Hesperidin significantly inhibited the expression of osteoclast-related genes, including NFATc1, CTSK, and TRAP(t=7.1, 4.8, 9.1, all P<0.05).

Conclusion

Hesperidin may inhibite titanium particles-mediated osteoclast differentiation and maturation.

Key words: Osteoclasts, Prosthesis failure, NFATC transcription factors

Jun Fan, Liping Cao. Hesperidin inhibits osteoclast differentiation mediated by titanium particles in vitro[J]. Chinese Journal of Joint Surgery(Electronic Edition), 2020, 14(06): 698-702.

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Figures/Tables 5

References 21

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