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Chinese Journal of Joint Surgery(Electronic Edition) ›› 2020, Vol. 14 ›› Issue (01): 63-67. doi: 10.3877/cma.j.issn.1674-134X.2020.01.011

Special Issue:

• Basic Research • Previous Articles     Next Articles

Primary research on biocompatibility of poly lactic acid modified bacterial cellulose

Hongxuan Huang1, Bo Bai1, Chen Lai2, Ying Wang3, Yi Chen1, Shujiang Zhang1,()   

  1. 1. Joint Surgery Department of the First Affiliated Hospital of Guangzhou Medical College, Guangdong Key Laboratory of Orthopedic Technology and Implanted Materials, Guangzhou 510120, China
    2. Shenzhen Key Laboratory of Human Tissue Regeneration and Repair, PKU-HKUST Shenzhen-Hongkong Institution, Shenzhen 518067, China
    3. Ultrasound Department of the First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China
  • Received:2018-05-13 Online:2020-02-01 Published:2020-02-01
  • Contact: Shujiang Zhang
  • About author:
    Corresponding author: Zhang Shujiang, Email:

Abstract:

Objective

To investigate the biocompatibility of poly lactic-co-glycolic acid (PLGA)modified bacterial cellulose using smooth muscle cells.

Methods

Different proportions of PLGA modified bacterial cellulose were divided into five groups: the pure PLGA group (the 50P group), bacterial cellulose modified by PLA ∶PGA=50 ∶50 as the 50PB group, bacterial cellulose with poly-lactic acid(PLA) ∶poly-glycolic acid(PGA)=75 ∶25 as the 75PB group and bacterial cellulose with PLA ∶PGA=90 ∶10 as the 90PB group; the pure bacterial cellulose as the control group. The smooth muscle cells were seeded to different kinds of materials and cultured for 7 d. The situation of smooth muscle cells on different materials was observed by electronic migroscope(SEM)and determined by live/dead staining. The cell proliferation on the materials was determined by cell counting kit-8(CCK-8) test. The data of cell survival rate and proliferation rate were analyzed by one-way ANOVA and SNK test.

Results

The smooth muscle cells in all the groups adhered well to the materials on the 3rd day according to SEM observation. The live/dead staining result showed that smooth muscle cells of all the groups distributed uniformly and grow flourishly(F=1.454, P>0.05). The CCK-8 data indicated that the growth of smooth muscle cells in different groups had no difference on the 3rd day and 5th day(3 d F=1.672, P>0.05; 5 d F=1.19, P>0.05). On the 7th day the cells both in the 50PB group and the control group showed superior proliferation to those in the 90PB group and the 75PB group(F=13.328, P<0.01).

Conclusion

The biocompatibility of PLGA (PLA ∶PGA=50 ∶50) modified bacterial cellulose is better than other PLGA proportion modified bacterial cellulose, which is potential biomaterial for tissue repair.

Key words: Cellulose, Polylactic acid-polyglycolic acid copolymer, Myocytes, smooth muscle, Biocompatible materials

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