单细胞测序技术在骨关节炎病因诊断中的研究进展

doi:10.3877/cma.j.issn.1674-134X.2022.01.007

骨关节炎是最常见的关节退行性病变之一，病因至今仍不明确，亟待进一步深入研究。单细胞测序技术通过单细胞分离、全基因组扩增和高通量测序等方法从单个细胞水平测序并分析细胞的遗传信息，对细胞进行来源分析和谱系追踪，在细胞水平上对骨关节炎的病理基础进行精准诊断与分型。将单细胞测序技术应用于骨关节炎的病因诊断已有长足进展，分离并鉴定了骨关节炎患者关节软骨、滑膜、软骨下骨中不同细胞簇，揭示其在骨关节炎疾病进展过程中参与的不同作用，为骨关节炎的预防与早期诊断提供了理论依据，在骨关节炎的治疗方面也有广阔的应用前景。

关键词: 单细胞分析, 骨关节炎, 病因学, 诊断

Osteoarthritis is one of the most common degenerative diseases of joints. As the etiology remains unclear, further research is urgently needed. Single-cell sequencing technology uses single-cell isolation, whole-genome amplification, and high-throughput sequencing to sequence and analyze the genetic information of cells at the level of individual cells, and perform source analysis and lineage tracing of cells. Accurate diagnosis and classification based on pathology. The application of single-cell sequencing technology to the diagnosis of the cause of osteoarthritis has made great progress. Different cell clusters in the articular cartilage, synovium, and subchondral bone of patients with osteoarthritis have been isolated and identified, revealing their role in the progression of osteoarthritis. The different roles of participation provide a theoretical basis for the prevention and early diagnosis of osteoarthritis, and it also has broad application prospects in the treatment of osteoarthritis.

Key words: Single-cell analysis, Osteoarthritis, Etiology, Diagnosis

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Research progress of single cell sequencing technology in etiological diagnosis of osteoarthritis

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