Application of next-generation sequencing technology in diagnosis of periprosthetic joint infection

doi:10.3877/cma.j.issn.1674-134X.2019.05.011

Abstract

Abstract:

Periprosthetic infection (PJI) is a disastrous complication after artificial joint replacement. In the treatment of PJI, early and timely diagnosis of PJI and identification of pathogenic microorganisms are still the keys to successful treatment. However, there are still many shortcomings in the existing bacterial culture technology. Next-generation sequencing technology has been gradually applied to the diagnosis of PJI due to its characteristics of rapid, accurate and comprehensive diagnosis of pathogenic microorganisms. But, most of the current clinical joint surgeons are still lack of understanding of the next-generation sequencing technology and its application in the diagnosis of PJI. This paper reviewed this issue.

Key words: Joint Prosthesis, Arthroplasty, Infection, Diagnosis

Yihong Xu, Shuo Li, Weidong Xu. Application of next-generation sequencing technology in diagnosis of periprosthetic joint infection[J]. Chinese Journal of Joint Surgery(Electronic Edition), 2019, 13(05): 584-588.

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方法	概括
16S扩增子靶向NGS	1.通过PCR反应扩增16S基因后的靶向NGS(主要为了避免宿主真核DNA提取的问题)
	2.将扩增的DNA聚合并装载到微球上进行反应，产生高水平的样本供进一步的NGS测序
	3.测序是在一个平台上进行的，每一个核苷酸结合到DNA中都会产生一个信号变化，然后对读取数据进行分析
	4.最后将序列与数据库进行比较，从而明确致病菌属级和种级的区别
鸟枪宏基因组测序	1.全基因组测序在混合微生物基因组中的应用
鸟枪宏基因组测序	2．DNA被提取出来，产生基因组的混合物，这些基因组被随机分割成一组随机的DNA序列，并与生物信息学序列数据库进行比对，明确致病菌

方法	概括
16S扩增子靶向NGS	1.通过PCR反应扩增16S基因后的靶向NGS(主要为了避免宿主真核DNA提取的问题)
	2.将扩增的DNA聚合并装载到微球上进行反应，产生高水平的样本供进一步的NGS测序
	3.测序是在一个平台上进行的，每一个核苷酸结合到DNA中都会产生一个信号变化，然后对读取数据进行分析
	4.最后将序列与数据库进行比较，从而明确致病菌属级和种级的区别
鸟枪宏基因组测序	1.全基因组测序在混合微生物基因组中的应用
鸟枪宏基因组测序	2．DNA被提取出来，产生基因组的混合物，这些基因组被随机分割成一组随机的DNA序列，并与生物信息学序列数据库进行比对，明确致病菌

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