Correlation between miRNA22-3p and miRNA223-3p expression and immune markers in gouty arthritis

doi:10.3877/cma.j.issn.1674-134X.2023.01.004

Abstract

Abstract:

Objective

To investigate the expression levels of miRNA22-3p and miRNA223-3p in gouty arthritis patients and the regulatory mechanism of inflammatory immune response.

Methods

The data of 80 gout patients who met the gouty diagnosis creteria and treated in the First Hospital of Qinhuangdao from January 2020 to January 2021 were retrospectively analyzed. Other 80 healthy patients who underwent rountine health check-up during the same period without obvious abnormality in clinical indicators were enrolled as the control group. Exclusion criteria: the patients with severe diseases of the vital organs and systems; patients who used hormone drugs in the past three months; secondary gouty arthritis caused by other diseases; dryg induced gouty arthritis attacks; mental disorders. The observation group met the diagnostic criteria of gout. Independent sample t test was used to compare the differences in miRNA22-3p, miRNA223-3p, infla-mmatory response and immune indicators between the two groups. Pearson correlation analysis was applied for the relationship between inflammatory indicators and miRNA22-3p as well as miRNA223-3p.

Results

Expression levels of miRNA22-3p and miRNA223-3p in the observation group were (4.01±1.03) and (1.51±0.12), respectively, which were significantly higher than those in the control group [(1.12±0.26) and (0.62±0.20)] (t=24.333, 34.130, both P<0.05). The levels of IL-1β, TNF-α and IL-6 in the observation group were (57.77±7.24), (24.04±4.24) and (49.27±3.72), respectively, which were significantly higher than those in the control group. (42.27±3.31), (18.07±4.17), (39.75±3.73), respectively (t=17.415, 8.979, 16.164, all P<0.05). The levels of monocyte chemoattractant protein (MCP)-1, thymus and activation regulated chemokine (TARC), eotaxin-2 in the observation group were(106±12), (215±13), (347±11) which were higher than those in the control group [(72±11), (159±12), (317±12)](t=18.095, 29.356, 16.713, all P<0.05). Pearson correlation analysis showed the expressions of miRNA22-3p and miRNA223-3p were positively correlated with MCP-1, TARC, eotaxin-2, IL-1β, TNF-α and IL-6 (r=0.609, 0.659, 0.545, 0.610, 0.551, 0.571, 0.684, 0.668, 0.671, 0.542, 0.534, 0.458, all P<0.05).

Conclusion

The expression levels of mirNA22-3p and mirNA223-3p in gouty arthritis patients are significantly increased, which are positively correlated with inflammation and immune response.

Key words: RNA, small untranslated, Arthritis, gouty, Cytokines

Lixin An, Qian Zhang, Kun Li, Zixu Liu, Jian Liu, Rui Shi. Correlation between miRNA22-3p and miRNA223-3p expression and immune markers in gouty arthritis[J]. Chinese Journal of Joint Surgery(Electronic Edition), 2023, 17(01): 29-34.

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URL: https://zhgjwkzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-134X.2023.01.004

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References 21

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组别Group	例数Case number	年龄Age[岁，(±s)]	身体质量指数Body mass index[kg/cm²，(±s)]	性别(男/女)Gender(male/female)
观察组Observation group	80	65.3±5.2	24.1±2.0	53/27
对照组Control group	80	65.3±2.1	24.1±2.5	58/22
统计值		t＝0.128	t＝0.028	χ²＝0.735
P值		>0.05	>0.05	>0.05

组别Group	例数Case number	年龄Age[岁，(±s)]	身体质量指数Body mass index[kg/cm²，(±s)]	性别(男/女)Gender(male/female)
观察组Observation group	80	65.3±5.2	24.1±2.0	53/27
对照组Control group	80	65.3±2.1	24.1±2.5	58/22
统计值		t＝0.128	t＝0.028	χ²＝0.735
P值		>0.05	>0.05	>0.05

组别Group	例数Case number	miRNA223-3p	miRNA22-3p
发作亚组Episodic subgroup	42	4.3±0.9	1.7±0.3
稳定亚组Stable subgroup	38	3.7±0.8	1.3±0.6
t值		3.237	3.600
P值		0.002	0.001

组别Group	例数Case number	miRNA223-3p	miRNA22-3p
发作亚组Episodic subgroup	42	4.3±0.9	1.7±0.3
稳定亚组Stable subgroup	38	3.7±0.8	1.3±0.6
t值		3.237	3.600
P值		0.002	0.001

组别Group	例数Case number	IL-1β(ng/L)	TNF-α(ng/L)	IL-6(ng/L)	NLRP3(相对表达量)	caspase(相对表达量)
观察组Observation group	80	57.77±7.24	24.04±4.24	49.27±3.72	8.56±2.43	7.11±1.14
对照组Control group	80	42.27±3.31	18.07±4.17	39.75±3.73	1.59±0.44	0.98±0.13
t值		17.415	8.979	16.164	25.244	47.785
P值		<0.001	<0.001	<0.001	<0.001	<0.001

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