切换至 "中华医学电子期刊资源库"
高级检索
中华关节外科杂志(电子版)

中华关节外科杂志(电子版) ›› 2020, Vol. 14 ›› Issue (02) : 196 -200. doi: 10.3877/cma.j.issn.1674-134X.2020.02.011

所属专题： 文献；

综述

中国富血小板血浆临床制备方法的研究进展
卫愉轩1, 张昭远2, 范峥莹2, 袁霆2,†()   
  1. 1. 518116　深圳，国家癌症中心/国家肿瘤临床医学研究中心/中国医学科学院北京协和医学院肿瘤医院深圳医院骨科
    2. 200233　上海交通大学附属第六人民医院骨科
  • 收稿日期:2019-10-31 出版日期:2020-04-01
  • 通信作者: 袁霆
  • 基金资助:
    国家自然科学基金(81572239); 上海申康医院发展中心临床科技创新项目(SHDC12016219)

Research progress of clinical preparation methods of platelet-rich plasma in China

Yuxuan Wei1, Zhaoyuan Zhang2, Zhengying Fan2, Ting Yuan2,†()   

  1. 1. Department of Orthopaedics, National Cancer Center/ National Clinical Research Center for Cancer/ Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Shenzhen 518116, China
    2. Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China
  • Received:2019-10-31 Published:2020-04-01
  • Corresponding author: Ting Yuan
  • About author:
    Corresponding author: Yuan Ting, Email:
全文 ( ) 下载PDF ( ) 导出引用
引用本文：

卫愉轩, 张昭远, 范峥莹, 袁霆. 中国富血小板血浆临床制备方法的研究进展[J/OL]. 中华关节外科杂志(电子版), 2020, 14(02): 196-200.

Yuxuan Wei, Zhaoyuan Zhang, Zhengying Fan, Ting Yuan. Research progress of clinical preparation methods of platelet-rich plasma in China[J/OL]. Chinese Journal of Joint Surgery(Electronic Edition), 2020, 14(02): 196-200.

自体富血小板血浆(PRP)是一种通过离心的方法从自体血中提取出来的血小板浓缩物。近20年来，已经被广泛应用于多种学科，如骨科、口腔颌面外科、整形美容科等。然而，至今，PRP的临床制作方法还没有统一标准，不同的制作方法导致PRP的成分以及临床疗效不同，给临床医务工作者应用PRP造成了诸多困扰。通过检索和分析中国已发表有关PRP制备的文献(中文和英文)发现，国内PRP临床制备方法差异性较大，包括采血量、离心设备、离心次数、离心力、离心时间和PRP制备量等。PRP中血小板富集度和回收率，白细胞浓度等指标也不稳定，亟需更高质量的研究或指南来规范和指导临床PRP的制备。

关键词: 富血小板血浆, 临床研究, 方法, 离心法

Autologous platelet-rich plasma (PRP) is platelet concentrate separated from autologous blood by centrifugation. In the past two decades, it has been widely used in various medical fields such as orthopedics, oral and maxillofacial surgery, plastic and cosmetic surgery. However, up to now, the consistent standard for PRP clinical preparation hasn’t established. Different preparation methods may lead to different components and clinical effects of PRP, which brings many problems for clinical practitioners to apply PRP. According the literatures (Chinese and English) on PRP preparation published in China, the parameters of domestic PRP preparation methods, including blood collection, centrifugation equipment, number of centrifugation, centrifugal force, centrifugation time and volume of PRP, are quite different. Other indicators of PRP, such as leukocyte concentration, platelet enrichment factor and recovery rate, are also unstable and most are not reptorted. Therefore, there is an urgent need for high-quality research or guidelines to standardize and guide the preparation of clinical PRP.

Key words: Platelet-rich plasma, Clinical study, Methods, Centrifugation
图1 中国已发表临床PRP(富血小板血浆)制备相关文献筛选流程图
[29]
Du L, Miao Y, Li X, et al. A novel and convenient method for the preparation and activation of PRP without any additives: temperature controlled PRP[J/OL]. Biomed Res Int，2018, 1761865. doi: 10.1155/2018/1761865.
[30]
Hosnuter M, Aslan C, Isik D, et al. Functional assessment of autologous platelet-rich plasma (PRP) after long-term storage at －20 ℃ without any preservation agent[J]. J Plast Surg Hand Surg, 2017, 51 (4): 235-239.
[31]
Hauschild G, Geburek F, Gosheger G, et al. Short term storage stability at room temperature of two different platelet-rich plasma preparations from equine donors and potential impact on growth factor concentrations[J/OL]. BMC Vet Res，2017，13 (1): 7. doi: 10.1186/s12917-016-0920-4.
[1]
Hsu WK, Mishra A, Rodeo SR, et al. Platelet-rich plasma in orthopaedic applications: evidence-based recommendations for treatment[J]. J Am Acad Orthop Surg, 2013, 21(12): 739-748.
[2]
袁霆，张长青，余楠生. 富血小板血浆在骨关节外科临床应用专家共识(2018年版)[J/CD]. 中华关节外科杂志(电子版), 2018，12 (05): 596-600.
[3]
邢丹，余楠生，袁霆，等．关节腔注射富血小板血浆治疗膝骨关节炎的临床实践指南(2018年版)[J/CD]．中华关节外科杂志(电子版)，2018，12(4): 444-448.
[4]
宣力，田举，宣敏，等．二次离心法制备富血小板血浆中血小板相关参数的分析[J]．华南国防医学杂志，2017，31(8): 514-517, 530.
[5]
王书军，温从吉，李诗言．不同套装制备的富血小板血浆中细胞及细胞因子成分的比较[J/CD]．中华关节外科杂志(电子版)，2016，10(6): 592-597.
[6]
Yin WJ, Xu ZL, Sheng JG, et al. Erythrocyte sedimentation rate and fibrinogen concentration of whole blood influences the cellular composition of platelet-rich plasma obtained from centrifugation methods[J]. Exp Ther Med, 2017, 14(3, A): 1909-1918.
[7]
Yin W, Xu H, Sheng J, et al. Optimization of pure platelet-rich plasma preparation: a comparative study of pure platelet-rich plasma obtained using different centrifugal conditions in a single-donor model[J]. Exp Ther Med, 2017, 14(3): 2060-2070.
[8]
Pietrzak WS, Eppley BL. Platelet rich plasma：biology and new technology[J]. J Craniofac Surg, 2005, 16(6): 1043-1054.
[9]
Marx RE. Platelet-rich plasma: evidence to support its use[J]. J Oral Maxillofac Surg, 2004, 62(4): 489-496.
[10]
Landesberg R, Roy M, Glickman RS. Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation[J]. J Oral Maxillofac Surg, 2000, 58(3): 297-300; discussion 300-301.
[11]
Marx RE. Platelet-rich plasma (PRP): what is PRP and what is not PRP？[J]. Implant Dent, 2001, 10(4): 225-228.
[12]
袁霆，张长青．骨组织及软组织修复作用中富血小板血浆的制作及其原理[J]．中国临床康复，2004，8(35): 7939-7941.
[13]
Ruf A, Patscheke H. Flow cytometric detection of activated platelets: comparison of determining shape change，fibrinogen binding，and P-selectin expression [J]. Semin Thromb Hemost, 1995, 21(2): 146-151.
[14]
Stenberg PE, Mcever RP, Shuman MA, et al. A platelet alpha-granule membrane protein (GMP-140) is expressed on the plasma membrane after activation [J]. J Cell Biol, 1985, 101(3): 880-886.
[15]
Di Y, Han C, Zhao L, et al. Is local platelet-rich plasma injection clinically superior to hyaluronic acid for treatment of knee osteoarthritis? A systematic review of randomized controlled trials[J/OL]. Arthritis Res Ther, 2018, 20(1): 128. doi: 10.1186/s13075-018-1621-0.
[16]
Shen LX, Yuan T, Chen SB, et al. The temporal effect of platelet-rich plasma on pain and physical function in the treatment of knee osteoarthritis: systematic review and meta-analysis of randomized controlled trials[J/OL]. J Orthop Surg Res, 2017, 12(1): doi: 10.1186/s13018-017-0521-3.
[17]
Boesen AP, Hansen R, Boesen MI, et al. Effect of high-volume injection, platelet-rich plasma, and sham treatment in chronic midportion achilles tendinopathy: a randomized double-blinded prospective study[J]. Am J Sports Med, 2017, 45(9): 2034-2043.
[18]
Albano D, Messina C, Usuelli FG, et al. Magnetic resonance and ultrasound in achilles tendinopathy: predictive role and response assessment to platelet-rich plasma and adipose-derived stromal vascular fraction injection[J]. Eur J Radiol, 2017, 95(95): 130-135.
[19]
Krogh TP, Ellingsen T, Christensen RA, et al. Ultrasound-guided injection therapy of achilles tendinopathy with platelet-rich plasma or saline: a randomized, blinded, placebo-controlled trial[J]. Am J Sports Med, 2016, 44(8): 1990-1997.
[20]
Miron RJ, Xu H, Chai J, et al. Comparison of platelet-rich fibrin (PRF) produced using 3 commercially available centrifuges at both high (～700 g) and low (～200 g) relative centrifugation forces[J]. Clin Oral Investig, 2020, 24 (3): 1171-1182.
[21]
Mariani E, Pulsatelli L. Platelet concentrates in musculoskeletal medicine[J/OL]. Int J Mol Sci, 2020, 21 (4): 1328. doi: 10.3390/ijms21041328.
[22]
Melo BAG, Luzo  CM, Lana J, et al. Centrifugation conditions in the L-PRP preparation affect soluble factors release and mesenchymal stem cell proliferation in fibrin nanofibers[J]. Molecules, 2019, 24 (15): 2729.
[23]
Chen Z, Deng Z, Ma Y, et al. Preparation, Procedures and evaluation of platelet-rich plasma injection in the treatment of knee osteoarthritis[J/OL]. J Vis Exp, 2019，(143): e57700.doi: 10.3791/57700.
[24]
Dohan Ehrenfest DM, Pinto NR, Pereda A, et al. The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors, and fibrin architecture of a leukocyte- and platelet-rich fibrin (L-PRF) clot and membrane[J]. Platelets，2018, 29 (2): 171-184.
[25]
de Melo BAG, Martins Shimojo AA, Marcelino Perez AG, et al. Distribution, recovery and concentration of platelets and leukocytes in L－PRP prepared by centrifugation[J]. Colloids Surf B Biointerfaces, 2018, 161: 288-295.
[26]
Piao L, Park H, Jo CH. Theoretical prediction and validation of cell recovery rates in preparing platelet-rich plasma through a centrifugation[J/OL]. PLoS One，2017，12 (11): e0187509.doi: 10.1371/journal.pone.0187509.
[27]
Steller D, Herbst N, Pries R, et al. Impact of incubation method on the release of growth factors in non-Ca(2＋)-activated PRP, Ca(2＋)-activated PRP, PRF and A-PRF[J]. J Craniomaxillofac Surg, 2019, 47 (2): 365-372.
[28]
Etulain J, Mena HA, Meiss RP, et al. An optimised protocol for platelet-rich plasma preparation to improve its angiogenic and regenerative properties[J]. Sci Rep, 2018, 8 (1): 1513.
[1] 曾铖, 张剑. 抗体药物偶联物在三阴性乳腺癌中的应用[J/OL]. 中华乳腺病杂志(电子版), 2024, 18(03): 140-145.
[2] 梁旭, 宋国红. 2023年激素受体阳性/HER-2阴性乳腺癌治疗的研究进展[J/OL]. 中华乳腺病杂志(电子版), 2024, 18(02): 71-77.
[3] 王雅楠, 刘丹, 曹正浓, 贾慧敏. 儿童迟发性先天性膈疝患儿的临床诊治特点分析[J/OL]. 中华妇幼临床医学杂志(电子版), 2024, 20(04): 410-419.
[4] 李亚龙, 王星童, 申传安. 异体富血小板血浆在创面修复中的临床应用进展[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(06): 541-545.
[5] 王振宇, 张洪美, 荆琳, 何名江, 闫奇. 膝骨关节炎相关炎症因子与血浆代谢物间的因果关系及中介效应[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(06): 467-473.
[6] 赵芊, 李亚坤, 李智. 同种异体富血小板血浆临床应用的研究进展[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(04): 364-367.
[7] 庹晓晔, 冯光. 复杂胸壁重建策略[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(03): 276-276.
[8] 苏永涛, 王春雷, 徐广琪, 关中正, 焦伟, 隋颖. 胫骨骨膜牵张术联合富血小板血浆对治疗糖尿病足溃疡的疗效观察[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(03): 238-244.
[9] 薛庆, 施赛叶, 徐雅文, 盛夏, 张芹芹. 追踪方法学联合失效模式与效应分析在膀胱灌注化疗患者中的应用[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2024, 18(06): 553-559.
[10] 阮星星, 黄智渊, 刘芙香, 狄金明. 从临床医师诊治患者的思路出发撰写临床研究论文[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2024, 18(04): 397-401.
[11] 嵇振岭, 陈杰, 唐健雄. 重视复杂腹壁疝手术并发症的预防和处理[J/OL]. 中华疝和腹壁外科杂志(电子版), 2024, 18(06): 601-606.
[12] 陈丽璇, 窦培宁, 肖扬. 干细胞治疗早发性卵巢功能不全的现状及未来展望[J/OL]. 中华细胞与干细胞杂志(电子版), 2024, 14(04): 239-248.
[13] 吉莉, 苏云楠, 王斌, 沈滔, 刘团结, 毛蕾, 徐玉萍, 张婷, 王博. 急性缺血性脑卒中患者脑白质微结构改变对长期认知功能损伤的预测价值研究[J/OL]. 中华脑科疾病与康复杂志(电子版), 2024, 14(04): 193-200.
[14] 郭义, 陈泽林, 赵雪, 杨毅, 徐泽成. 中医针灸标准化基本理论和方法的探索与实践[J/OL]. 中华针灸电子杂志, 2024, 13(04): 133-139.
[15] 欧阳川, 朱巧珍, 欧阳林. 腰椎间盘退变的生物代谢特征及评价技术研究进展[J/OL]. 中华诊断学电子杂志, 2024, 12(03): 206-211.
阅读次数
全文


摘要

版权所有 中华医学会 中华医学电子音像出版社有限责任公司  京ICP备14006079号-1  网络出版服务许可证：(署)网出证(京)字第075号