Abstract:
Objective
To establish a New Zealand rabbit distraction osteogenesis model， and to investigate the effect of total flavonoids of drynaria rhizome on spinal bone volume of distraction osteogenesis model by Micro-CT bone microstructure indices.
Methods
Twelve New Zealand male rabbits， weighing 1.8 to 2.2 kg， were randomly divided into three groups according to the random number table method： the blank group， the control group and the experimental group， with four animals in each group. The control and experimental groups were subjected to distraction osteogenesis modelling， and the blank group were subjected to sham operation. After the operation， the experimental group were given total flavonoids of drynaria rhizome（0.03g·kg-1·d-1 ） ， and the other two groups were given equal amounts of saline lavage for 60 d. After the animal was sacrificed， the same spinal bones were removed and scanned by micro-CT. Bone mineral density （BMD），bone volume fraction （BV/TV）， trabecular separation （Tb.Sp）， trabecular thickness （Tb.Th）， trabecular gap （Tb.N） and bone surface area to bone volume ratio （BS/BV） were measured. One-way ANOVA was used to analyse the data of the three groups， and LSD test was used for multiple comparisons.
Results
All the rabbits were observed and showed normal activities after operation. The rabbits in the control and experimental groups showed a decrease in running and jumping. Quantitative analysis of micro-CT showed statistically significant differences in BMD， BS/BV， Tb.Th， Tb.N， BV/TV， and Tb.Sp among the three groups （F=3.751， 3.141， 8.590， 4.037， 4.133，5.110， all P＜0.05）. BMD， Tb.Th， Tb.N， BV/TV decreased， and BS/BV and Tb.Sp increased in the control group compared with the blank group （all P＜0.05）. Compared with the experimental group， BMD， Tb.Th， Tb.N， BV/TV decreased and Tb.Sp increased in the control group （all P＜0.05）； the difference was not statistically significant in the blank group compared with the experimental group （all P ＞0.05）.
Conclusions
Bone mass may decrease after distraction osteogenesis， this may lead to a risk of osteoporosis. The total flavones of drynaria rhizome can maintain the spine bone mass of distraction osteogenesis model and prevent the occurrence of osteoporosis.
Key words:
Flavonoids,
Osteogenesis,
distraction,
Osteoporosis,
X-ray microtomography
Yongle Zheng, Zucai Pang, Jiamin Chen, Bingyin Sun. Study on impact of total flavonoids from drynaria rhizome on osteoporosis in distraction osteogenesis model[J]. Chinese Journal of Joint Surgery(Electronic Edition), 2024, 18(05): 604-608.