切换至 "中华医学电子期刊资源库"
高级检索
中华关节外科杂志(电子版)

中华关节外科杂志(电子版) ›› 2020, Vol. 14 ›› Issue (02) : 196 -200. doi: 10.3877/cma.j.issn.1674-134X.2020.02.011

所属专题： 文献；

综述

中国富血小板血浆临床制备方法的研究进展
卫愉轩1, 张昭远2, 范峥莹2, 袁霆2,†()   
  1. 1. 518116　深圳，国家癌症中心/国家肿瘤临床医学研究中心/中国医学科学院北京协和医学院肿瘤医院深圳医院骨科
    2. 200233　上海交通大学附属第六人民医院骨科
  • 收稿日期:2019-10-31 出版日期:2020-04-01
  • 通信作者: 袁霆
  • 基金资助:
    国家自然科学基金(81572239); 上海申康医院发展中心临床科技创新项目(SHDC12016219)

Research progress of clinical preparation methods of platelet-rich plasma in China

Yuxuan Wei1, Zhaoyuan Zhang2, Zhengying Fan2, Ting Yuan2,†()   

  1. 1. Department of Orthopaedics, National Cancer Center/ National Clinical Research Center for Cancer/ Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Shenzhen 518116, China
    2. Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China
  • Received:2019-10-31 Published:2020-04-01
  • Corresponding author: Ting Yuan
  • About author:
    Corresponding author: Yuan Ting, Email:
全文 ( ) 下载PDF ( ) 导出引用
引用本文：

卫愉轩, 张昭远, 范峥莹, 袁霆. 中国富血小板血浆临床制备方法的研究进展[J]. 中华关节外科杂志(电子版), 2020, 14(02): 196-200.

Yuxuan Wei, Zhaoyuan Zhang, Zhengying Fan, Ting Yuan. Research progress of clinical preparation methods of platelet-rich plasma in China[J]. Chinese Journal of Joint Surgery(Electronic Edition), 2020, 14(02): 196-200.

自体富血小板血浆(PRP)是一种通过离心的方法从自体血中提取出来的血小板浓缩物。近20年来，已经被广泛应用于多种学科，如骨科、口腔颌面外科、整形美容科等。然而，至今，PRP的临床制作方法还没有统一标准，不同的制作方法导致PRP的成分以及临床疗效不同，给临床医务工作者应用PRP造成了诸多困扰。通过检索和分析中国已发表有关PRP制备的文献(中文和英文)发现，国内PRP临床制备方法差异性较大，包括采血量、离心设备、离心次数、离心力、离心时间和PRP制备量等。PRP中血小板富集度和回收率，白细胞浓度等指标也不稳定，亟需更高质量的研究或指南来规范和指导临床PRP的制备。

关键词: 富血小板血浆, 临床研究, 方法, 离心法

Autologous platelet-rich plasma (PRP) is platelet concentrate separated from autologous blood by centrifugation. In the past two decades, it has been widely used in various medical fields such as orthopedics, oral and maxillofacial surgery, plastic and cosmetic surgery. However, up to now, the consistent standard for PRP clinical preparation hasn’t established. Different preparation methods may lead to different components and clinical effects of PRP, which brings many problems for clinical practitioners to apply PRP. According the literatures (Chinese and English) on PRP preparation published in China, the parameters of domestic PRP preparation methods, including blood collection, centrifugation equipment, number of centrifugation, centrifugal force, centrifugation time and volume of PRP, are quite different. Other indicators of PRP, such as leukocyte concentration, platelet enrichment factor and recovery rate, are also unstable and most are not reptorted. Therefore, there is an urgent need for high-quality research or guidelines to standardize and guide the preparation of clinical PRP.

Key words: Platelet-rich plasma, Clinical study, Methods, Centrifugation
图1 中国已发表临床PRP(富血小板血浆)制备相关文献筛选流程图
[29]
Du L, Miao Y, Li X, et al. A novel and convenient method for the preparation and activation of PRP without any additives: temperature controlled PRP[J/OL]. Biomed Res Int，2018, 1761865. doi: 10.1155/2018/1761865.
[30]
Hosnuter M, Aslan C, Isik D, et al. Functional assessment of autologous platelet-rich plasma (PRP) after long-term storage at －20 ℃ without any preservation agent[J]. J Plast Surg Hand Surg, 2017, 51 (4): 235-239.
[31]
Hauschild G, Geburek F, Gosheger G, et al. Short term storage stability at room temperature of two different platelet-rich plasma preparations from equine donors and potential impact on growth factor concentrations[J/OL]. BMC Vet Res，2017，13 (1): 7. doi: 10.1186/s12917-016-0920-4.
[1]
Hsu WK, Mishra A, Rodeo SR, et al. Platelet-rich plasma in orthopaedic applications: evidence-based recommendations for treatment[J]. J Am Acad Orthop Surg, 2013, 21(12): 739-748.
[2]
袁霆，张长青，余楠生. 富血小板血浆在骨关节外科临床应用专家共识(2018年版)[J/CD]. 中华关节外科杂志(电子版), 2018，12 (05): 596-600.
[3]
邢丹，余楠生，袁霆，等．关节腔注射富血小板血浆治疗膝骨关节炎的临床实践指南(2018年版)[J/CD]．中华关节外科杂志(电子版)，2018，12(4): 444-448.
[4]
宣力，田举，宣敏，等．二次离心法制备富血小板血浆中血小板相关参数的分析[J]．华南国防医学杂志，2017，31(8): 514-517, 530.
[5]
王书军，温从吉，李诗言．不同套装制备的富血小板血浆中细胞及细胞因子成分的比较[J/CD]．中华关节外科杂志(电子版)，2016，10(6): 592-597.
[6]
Yin WJ, Xu ZL, Sheng JG, et al. Erythrocyte sedimentation rate and fibrinogen concentration of whole blood influences the cellular composition of platelet-rich plasma obtained from centrifugation methods[J]. Exp Ther Med, 2017, 14(3, A): 1909-1918.
[7]
Yin W, Xu H, Sheng J, et al. Optimization of pure platelet-rich plasma preparation: a comparative study of pure platelet-rich plasma obtained using different centrifugal conditions in a single-donor model[J]. Exp Ther Med, 2017, 14(3): 2060-2070.
[8]
Pietrzak WS, Eppley BL. Platelet rich plasma：biology and new technology[J]. J Craniofac Surg, 2005, 16(6): 1043-1054.
[9]
Marx RE. Platelet-rich plasma: evidence to support its use[J]. J Oral Maxillofac Surg, 2004, 62(4): 489-496.
[10]
Landesberg R, Roy M, Glickman RS. Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation[J]. J Oral Maxillofac Surg, 2000, 58(3): 297-300; discussion 300-301.
[11]
Marx RE. Platelet-rich plasma (PRP): what is PRP and what is not PRP？[J]. Implant Dent, 2001, 10(4): 225-228.
[12]
袁霆，张长青．骨组织及软组织修复作用中富血小板血浆的制作及其原理[J]．中国临床康复，2004，8(35): 7939-7941.
[13]
Ruf A, Patscheke H. Flow cytometric detection of activated platelets: comparison of determining shape change，fibrinogen binding，and P-selectin expression [J]. Semin Thromb Hemost, 1995, 21(2): 146-151.
[14]
Stenberg PE, Mcever RP, Shuman MA, et al. A platelet alpha-granule membrane protein (GMP-140) is expressed on the plasma membrane after activation [J]. J Cell Biol, 1985, 101(3): 880-886.
[15]
Di Y, Han C, Zhao L, et al. Is local platelet-rich plasma injection clinically superior to hyaluronic acid for treatment of knee osteoarthritis? A systematic review of randomized controlled trials[J/OL]. Arthritis Res Ther, 2018, 20(1): 128. doi: 10.1186/s13075-018-1621-0.
[16]
Shen LX, Yuan T, Chen SB, et al. The temporal effect of platelet-rich plasma on pain and physical function in the treatment of knee osteoarthritis: systematic review and meta-analysis of randomized controlled trials[J/OL]. J Orthop Surg Res, 2017, 12(1): doi: 10.1186/s13018-017-0521-3.
[17]
Boesen AP, Hansen R, Boesen MI, et al. Effect of high-volume injection, platelet-rich plasma, and sham treatment in chronic midportion achilles tendinopathy: a randomized double-blinded prospective study[J]. Am J Sports Med, 2017, 45(9): 2034-2043.
[18]
Albano D, Messina C, Usuelli FG, et al. Magnetic resonance and ultrasound in achilles tendinopathy: predictive role and response assessment to platelet-rich plasma and adipose-derived stromal vascular fraction injection[J]. Eur J Radiol, 2017, 95(95): 130-135.
[19]
Krogh TP, Ellingsen T, Christensen RA, et al. Ultrasound-guided injection therapy of achilles tendinopathy with platelet-rich plasma or saline: a randomized, blinded, placebo-controlled trial[J]. Am J Sports Med, 2016, 44(8): 1990-1997.
[20]
Miron RJ, Xu H, Chai J, et al. Comparison of platelet-rich fibrin (PRF) produced using 3 commercially available centrifuges at both high (～700 g) and low (～200 g) relative centrifugation forces[J]. Clin Oral Investig, 2020, 24 (3): 1171-1182.
[21]
Mariani E, Pulsatelli L. Platelet concentrates in musculoskeletal medicine[J/OL]. Int J Mol Sci, 2020, 21 (4): 1328. doi: 10.3390/ijms21041328.
[22]
Melo BAG, Luzo  CM, Lana J, et al. Centrifugation conditions in the L-PRP preparation affect soluble factors release and mesenchymal stem cell proliferation in fibrin nanofibers[J]. Molecules, 2019, 24 (15): 2729.
[23]
Chen Z, Deng Z, Ma Y, et al. Preparation, Procedures and evaluation of platelet-rich plasma injection in the treatment of knee osteoarthritis[J/OL]. J Vis Exp, 2019，(143): e57700.doi: 10.3791/57700.
[24]
Dohan Ehrenfest DM, Pinto NR, Pereda A, et al. The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors, and fibrin architecture of a leukocyte- and platelet-rich fibrin (L-PRF) clot and membrane[J]. Platelets，2018, 29 (2): 171-184.
[25]
de Melo BAG, Martins Shimojo AA, Marcelino Perez AG, et al. Distribution, recovery and concentration of platelets and leukocytes in L－PRP prepared by centrifugation[J]. Colloids Surf B Biointerfaces, 2018, 161: 288-295.
[26]
Piao L, Park H, Jo CH. Theoretical prediction and validation of cell recovery rates in preparing platelet-rich plasma through a centrifugation[J/OL]. PLoS One，2017，12 (11): e0187509.doi: 10.1371/journal.pone.0187509.
[27]
Steller D, Herbst N, Pries R, et al. Impact of incubation method on the release of growth factors in non-Ca(2＋)-activated PRP, Ca(2＋)-activated PRP, PRF and A-PRF[J]. J Craniomaxillofac Surg, 2019, 47 (2): 365-372.
[28]
Etulain J, Mena HA, Meiss RP, et al. An optimised protocol for platelet-rich plasma preparation to improve its angiogenic and regenerative properties[J]. Sci Rep, 2018, 8 (1): 1513.
[1] 刘佳璇, 徐兵河. 中国乳腺癌临床研究年度进展[J]. 中华乳腺病杂志(电子版), 2023, 17(05): 259-265.
[2] 贺敬龙, 尚宏喜, 郝敏, 谢伟, 高明宏, 孙炜, 刘安庆. 重度类风湿关节炎患者行多关节置换术的临床手术疗效[J]. 中华关节外科杂志(电子版), 2023, 17(06): 860-864.
[3] 夏传龙, 迟健, 丛强, 连杰, 崔峻, 陈彦玲. 富血小板血浆联合关节镜治疗半月板损伤的临床疗效[J]. 中华关节外科杂志(电子版), 2023, 17(06): 877-881.
[4] 欧阳剑锋, 李炳权, 叶永恒, 胡少宇, 向阳. 关节镜联合富血小板血浆治疗粘连性肩周炎的疗效[J]. 中华关节外科杂志(电子版), 2023, 17(06): 765-772.
[5] 许正文, 李振, 侯振扬, 苏长征, 朱彪. 富血小板血浆联合植骨治疗早期非创伤性股骨头坏死[J]. 中华关节外科杂志(电子版), 2023, 17(06): 773-779.
[6] 张中斌, 付琨朋, 朱凯, 张玉, 李华. 胫骨高位截骨术与富血小板血浆治疗膝骨关节炎的疗效[J]. 中华关节外科杂志(电子版), 2023, 17(05): 633-641.
[7] 王泽勇, 覃健. 白细胞含量对富血小板血浆治疗运动系统损伤的影响[J]. 中华关节外科杂志(电子版), 2023, 17(05): 684-688.
[8] 詹钦文, 靳科, 袁家钦. 不同浓度自体富血小板血浆对慢性跟腱损伤的疗效[J]. 中华关节外科杂志(电子版), 2023, 17(04): 500-507.
[9] 王璐, 樊杨. 子宫内膜癌相关生物标志物研究现状[J]. 中华妇幼临床医学杂志(电子版), 2023, 19(05): 511-516.
[10] 韩李念, 王君. 放射性皮肤损伤治疗的研究进展[J]. 中华损伤与修复杂志(电子版), 2023, 18(06): 533-537.
[11] 姚宏伟, 魏鹏宇, 高加勒, 张忠涛. 不断提高腹腔镜右半结肠癌D3根治术的规范化[J]. 中华普外科手术学杂志(电子版), 2024, 18(01): 1-4.
[12] 任加发, 邬步云, 邢昌赢, 毛慧娟. 2022年急性肾损伤领域基础与临床研究进展[J]. 中华肾病研究电子杂志, 2023, 12(05): 276-281.
[13] 马聪, 李雪靖, 郑晓佐, 张晓阳, 段坤峰, 刘国强, 郄素会. 关节腔内注射富血小板血浆与透明质酸钠治疗Ⅰ-Ⅲ期膝骨关节炎的对比研究[J]. 中华老年骨科与康复电子杂志, 2023, 09(05): 282-288.
[14] 郑景达, 黄金旗, 张庆贤, 陈煌, 高晓杰. 小腿深静脉入路AngioJet一站式血栓清除治疗全肢型急性下肢深静脉血栓形成患者的临床研究[J]. 中华介入放射学电子杂志, 2023, 11(04): 305-309.
[15] 沈袁恒. 医学检验实验室自建检测方法的现状分析与管理展望[J]. 中华临床实验室管理电子杂志, 2023, 11(04): 200-206.
阅读次数
全文


摘要

版权所有 中华医学会 中华医学电子音像出版社有限责任公司  京ICP备14006079号-1  网络出版服务许可证：(署)网出证(京)字第075号